Identify members of putative gene families using BLAST-based
prediction strategies from proteins of Andropogoneae species
BLASTP-based gene family member identification can be performed directly using the HEMU BLAST module.
Below are instructions on curating reference protein sequence and performing the BLASTP search.
First, one should obtain representative sequence from a protein family for member identification in other species.
Reference sequence can be curated manually, or downloaded from a publicly-available RefSeq database.
Set a rational E-value (like 1E-20) and perform the protein-protein BLAST.